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Image Search Results
Journal: Journal of cellular and molecular medicine
Article Title: Functional analysis of microRNAs in human hepatocellular cancer stem cells.
doi: 10.1111/j.1582-4934.2011.01282.x
Figure Lengend Snippet: Fig. 1 Characterization of Cancer Stem Cells from human HCC tissues. (A) Real-time analysis of Oct 4, CD 133, Nestin, telom- erase, SSEA-4, AFP and CEA mRNA expres- sion in HCC stem cell under self-renewal con- dition (HSC-SR), under differentiation (HSC- DF) and HepG2 HCC cells. All the markers are significantly up-regulated in HCC cancer stem cells. *P 0.05 when compared with HepG2 group. (B) FACS analysis of HCC stem cells on day 1 before cell sorting. Results are given as the percentage of Oct-4 and CD133
Article Snippet: Human hepatocellular CSCs and
Techniques: FACS
Journal: Journal of cellular and molecular medicine
Article Title: Functional analysis of microRNAs in human hepatocellular cancer stem cells.
doi: 10.1111/j.1582-4934.2011.01282.x
Figure Lengend Snippet: Fig. 4 HCC stem cells are resistant to con- ventional chemotherapy. (A, C) After serum starvation of cultured cells overnight, sorafenib and doxorubicin were added at various concentrations (10 3–10 10 M) and cell viability was assessed after 72 hrs. IC50 graph of high-content image analysis of human hepatocytes and HCC stem cells treated by sorafenib and doxorubicin was illustrated. Quantitative data of MTS assay in the sorafenib treated cells were calcu- lated for IC50 with XLfit software, which is marked in the middle of the box. (B, D) IC50 of sorafenib and doxorubicin in hepato- cytes and HCC stem cells is illustrated in bar graph. HCC stem cells under either self- renewal (HSC-SR) or differentiation (HSC- DF) are more resistance to sorafenib and doxorubicin than HepG2 cells and normal liver stem cells. IC50 results are expressed as the mean S.E. of eight different exper- iments. (E) Groups of five SCID Berge mice were selected 8–10 weeks old per treat- ment group. The human HSC-SRs (1000) as well as parental HCC cells (5 106) were injected subcutaneously and allowed to engraft for 10 days. At day 10 the tumour was visible, and then the doxorubicin treat- ment was started and the mice received the desired concentration of the drug intraper- oteneal three times per week for 14 days. At the end of the 14 days the tumour was dis- sociated into single cell suspension and assayed with Almar Blue to determine per- cent inhibition of the drug. (F, G) Differential expression of CD133 and let-7a in vivo following treatment with doxorubicin by real-time PCR is shown. Data represent percentage change in expression in doxo- cibicin-treated tumours compared with controls. *P 0.05 relative to controls.
Article Snippet: Human hepatocellular CSCs and
Techniques: Cell Culture, MTS Assay, Software, Injection, Concentration Assay, Suspension, Inhibition, Quantitative Proteomics, In Vivo, Real-time Polymerase Chain Reaction, Expressing